{"id":7,"date":"2021-02-11T19:29:18","date_gmt":"2021-02-11T19:29:18","guid":{"rendered":"http:\/\/stopagingstemcells.com\/?p=7"},"modified":"2021-02-11T19:29:30","modified_gmt":"2021-02-11T19:29:30","slug":"single-cell-transcriptome-analysis-of-uncultured-human-umbilical-cord-mesenchymal-stem-cells","status":"publish","type":"post","link":"https:\/\/stopagingstemcells.com\/index.php\/2021\/02\/11\/single-cell-transcriptome-analysis-of-uncultured-human-umbilical-cord-mesenchymal-stem-cells\/","title":{"rendered":"Single-cell transcriptome analysis of uncultured human umbilical cord mesenchymal stem cells"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large\"><img fetchpriority=\"high\" decoding=\"async\" width=\"624\" height=\"347\" src=\"http:\/\/stopagingstemcells.com\/wp-content\/uploads\/2021\/02\/image.png\" alt=\"\" class=\"wp-image-8\" srcset=\"https:\/\/stopagingstemcells.com\/wp-content\/uploads\/2021\/02\/image.png 624w, https:\/\/stopagingstemcells.com\/wp-content\/uploads\/2021\/02\/image-300x167.png 300w\" sizes=\"(max-width: 624px) 100vw, 624px\" \/><\/figure>\n\n\n\n<ul class=\"wp-block-list\"><li><a href=\"javascript:;\">Shaoyang Zhang<\/a>,&nbsp;<a href=\"javascript:;\">Jing Yi Wang<\/a>,&nbsp;<a href=\"javascript:;\">Baojie Li<\/a>,&nbsp;<a href=\"javascript:;\">Feng Yin<\/a>&nbsp;&amp;&nbsp;<a href=\"javascript:;\">Huijuan Liu<\/a>&nbsp;<\/li><\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/stemcellres.biomedcentral.com\/\"><em>Stem Cell Research &amp; Therapy<\/em><\/a>&nbsp;<strong>volume<\/strong><strong>&nbsp;12<\/strong>, Article&nbsp;number:&nbsp;25&nbsp;(2021)&nbsp;<a href=\"https:\/\/stemcellres.biomedcentral.com\/articles\/10.1186\/s13287-020-02055-1#citeas\">Cite this article<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Abstract<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Umbilical cord mesenchymal stem cells (UC-MSCs) have certain advantages over other MSCs and about 300 clinical trials have been registered using UC-MSCs to treat diseases such as osteoarthritis, autoimmune diseases, and degenerative disorders, yet, only limited success has been achieved. One reason is that in vitro expanded UC-MSCs show tremendous heterogeneity and their relationship to in vivo UC-MSCs remains unknown. Here, we investigated freshly isolated, uncultured UC-MSCs by single-cell RNA sequencing (scRNA-seq) and found two populations of UC-MSCs. Although UC-MSCs share many expressed genes and may have the same origin, they can be clearly separated based on differentially expressed genes including CD73 and other markers. Moreover, group 1 MSCs are enriched in expression of genes in immune response\/regulatory activities, muscle cell proliferation and differentiation, stemness, and oxidative stress while group 2 MSCs are enriched with gene expression in extracellular matrix production, osteoblast and chondrocytes differentiation, and bone and cartilage growth. These findings suggest that UC-MSCs should be separated right after isolation and individually expanded in vitro to treat different diseases.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">READ MORE:&nbsp; https:\/\/stemcellres.biomedcentral.com\/articles\/10.1186\/s13287-020-02055-1<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Shaoyang Zhang,&nbsp;Jing Yi Wang,&nbsp;Baojie Li,&nbsp;Feng Yin&nbsp;&amp;&nbsp;Huijuan Liu&nbsp; Stem Cell Research &amp; Therapy&nbsp;volume&nbsp;12, Article&nbsp;number:&nbsp;25&nbsp;(2021)&nbsp;Cite this article Abstract Umbilical cord mesenchymal stem cells (UC-MSCs) have certain advantages over other MSCs and about 300 clinical trials have been registered using UC-MSCs to treat diseases such as osteoarthritis, autoimmune diseases, and degenerative disorders, yet, only limited success has been [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":8,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center 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